The Mildred Scheel Young Investigators Centre (MSNZ) P² at the Faculty of Medicine Carl Gustav Carus Dresden, University of Technology promotes young scientists in translational cancer research. The programme, which is funded by the German Cancer Aid, is linked to the NCT/UCC of the University Hospital and supports the establishment of junior oncology research groups on the Dresden campus. The running projects are focussing to optimise oncological therapy approaches in the long term. The research group “CRISPR/Cas9 genetic screens in 3D organoid culture as a means to uncover resistance marker genes in gastrointestinal cancers” is looking for
Technical Assistant (MTA/ BTA/ CTA) starting at 1st of June 2021
(full-time employment; limited for an initial period of 12 months; salary according TV-L)
The planned activity will be involved in the translational project on the investigation of drug resistance in gastric carcinomas. The main objective is the generation of resistant, gastrointestinal 3D organoid models and the functional analysis of resistance processes on molecular level. Your main activity is the establishment, maintenance as well as drug treatment of 3D organoid cultures generated from patient material. In order to identify the resistance-causing genes, the organoid cultures are analysed molecularly before and after resistance detection followed by a functional analysis of the candidate genes found using comprehensive CRISPR/Cas9 screens.
Due to the use of lentiviruses, work in the S2 genetic area is required. Within your work you will support the scientific staff in their research projects, but you are also expected to carry out activities on your own responsibility. A training of the innovative range of methods and later independent application of these methods is expressly desired and supported. In addition, you will also carry out administrative work, such as ordering reagents and consumables.
- successfully completed training as an MTA/BTA/CTA with state recognition
- experience with cell biological work under R1/S1 and R1/S2 conditions in vitro and/or in vivo
- Experience with working techniques such as post-processing of human sample material for use in culture and histology, cell culture (cell lines, primary cultures), immunohistochemical staining
- Familiar with standard (molecular biological) methods such as RNA and DNA isolation, PCRs and quantitative PCR, Western blot, cloning
- Experience with FACS analysis is desirable
- Ideally experience with lentiviral transduction
- Very good knowledge of written and spoken English (working in an international environment)
- Reliability, flexibility, organisational skills and good communication skills
- Independent and structured way of working
We offer you the opportunity to:
- Implement your own ideas and work in an innovative interdisciplinary team
- Family friendly arrangement of flexible working hours
- Care for your children through partnerships with children’s facilities in the vicinity of the University Hospital
- Use of company prevention offers, courses and fitness in our health centre Carus Vital
- Participation in job-oriented training, further education and continuing education opportunities at our Carus Academy
- Provision for the time after active employment in the form of a company pension scheme
- Use of our job ticket for public transport in Dresden and the surrounding area
Severely disabled persons are expressly invited to apply.
We kindly ask you to apply preferably online in order to make the personnel selection process faster and more effective. Of course, we will also process your written applications (with a stamped return envelope) without any disadvantages for you.
We look forward to receiving your application documents, which you should send to us online by 30 April 2021 under the reference number MSN0221508.