The Mildred Scheel Junior Research Center (MSNZ) P² at the Medical Faculty of the Dresden University of Technology promotes young scientists in translational cancer research. The program, which is funded by the German Cancer Aid, is affiliated to the NCT / UCC of the University Hospital and supports the establishment of junior research groups in the scientific field of oncology on the Dresden campus. The projects are addressing clinic-related questions with the aim to optimize oncological therapeutic approaches on a long-term basis. The research group with the topic “CRISPR / Cas9 genetic screens in 3D organoid culture as a means to uncover resistance markers genes in gastrointestinal cancers” is looking for a
(full time position / payment based on TV-L – E9 / initially limited to 3 years)
at the earliest possible starting date.
The planned activity will be integrated in a translational project investigating drug resistance in gastric carcinomas. The main goal of the project is the generation of resistant gastrointestinal 3D organoid models and the functional analysis of resistance mechanism on a molecular level.
The candidate’s main activity will be the establishment, maintenance and drug treatment of 3D organoid cultures generated from patient material. Functional analysis will follow using comprehensive CRISPR / Cas9 screens. The experimental use of lentiviruses will require lab work in a S2 laboratory.
The candidate will support the scientific staff in their research projects, but should also take over responsibilities and work independently. The candidate will be trained with the innovative and varied spectrum of methods to enable an independent working. In addition, the candidate will fulfill administrative tasks, such as the ordering of reagents and consumables.
- Completed training as BTA / MTA / CTA
- Experience with cell biological experiments (in vitro and / or in vivo) under R1/S1 and R1/S2 conditions
- Experience with handling human sample material for its use in culture and histology, cell culture (cell lines, primary cultures), immunohistochemical staining
- Comprehensive knowledge of (molecular biological) standard methods such as RNA and DNA isolation, PCRs and quantitative PCR, Western Blot, cloning
- Experiences with FACS analysis is desirable
- Experience and knowledge of lentiviral transduction methods would be ideal
- Very good spoken and written English (working in an international team)
- Reliability, flexibility, organizational skills and good communication skills
- Responsible and structured way of working
- Activity in the leading medical research associated with a highly specialized work environment
- Work in an innovative interdisciplinary team
- Flexibility in working hours to better combine work and family
- Child care through partnerships with day care centers near the University Hospital
- Prevention offers, courses and fitness in our health center Carus Vital
- Participation in job-oriented trainings, education opportunities in our Carus Academy
- A company pension plan
- Use of our job ticket for public transport in Dresden and surrounding regions
Female candidates are expressly invited to submit their application: the same also applies to disabled people.
Applications received by 18th of August 2019 will get full consideration. Please submit your detailed strongly stated application (reference: MSN0119706) as one PDF-file via the online portal of the University Hospital Carl Gustav Carus:
In case of questions please contact Dr. Ing. Jovan Mircetic and Dr. Ing. Peggy Jungke at 0351-463-40278 and 0351-458-11779 or by e-mail: firstname.lastname@example.org and email@example.com