Most important publications

Mukhametzyanova L, Schmitt LT, Torres-Rivera J, Rojo-Romanos T, Lansing F, Paszkowski-Rogacz M, Hollak H, Brux M, Augsburg M, Schneider PM, Buchholz F (2024). Activation of recombinases at specific DNA loci by zinc-finger domain insertions. Nature Biotechnology. 2024 Jan 31. doi: 10.1038/s41587-023-02121-y. PubMed | Nature Biotechnology

Schmitt LT, Schneider A, Posorski J, Lansing F, Jelicic M, Jain M, Sayed S, Buchholz F, Sürün D (2023). Quantification of evolved DNA-editing enzymes at scale with DEQSeq. Genome Biol. 2023 Nov 6;24(1):254.  doi: 10.1186/s13059-023-03097-3. PubMed

Rojo-Romanos T, Karpinski J, Millen S, Beschorner N, Simon F, Paszkowski-Rogacz M, Lansing F, Schneider PM, Sonntag J, Hauber J, Thoma-Kress AK, Buchholz F (2023). Precise excision of HTLV-1 provirus with a designer-recombinase. Mol Ther. 2023 Mar 17:S1525-0016(23)00135-1. doi: 10.1016/j.ymthe.2023.03.014. Online ahead of print. PubMed

Sayed S, Sidorova OA, Hennig A, Augsburg M, Cortes Vesga CP, Abohawya M, Schmitt LT, Sürün D, Stange DE, Mircetic J, Buchholz F (2022). Efficient correction of oncogenic KRAS and TP53 mutations through CRISPR base editing. Cancer Res. 2022 Jul 8;can.21.2519. doi: 10.1158/0008-5472.CAN-21-2519. PubMed

Lansing F, Mukhametzyanova L, Rojo-Romanos T, Iwasawa K, Kimura M, Paszkowski-Rogacz M, Karpinski J, Grass T, Sonntag J, Schneider PM, Günes C, Hoersten J, Schmitt LT, Rodriguez-Muela N, Knöfler R, Takebe T & Buchholz F (2022). Correction of a Factor VIII genomic inversion with designer-recombinases. Nature Communications volume 13, Article number: 422. doi: 10.1038/s41467-022-28080-7. PubMed

Hoersten J, Ruiz-Gómez G, Lansing F, Rojo-Romanos T, Schmitt LT, Sonntag J, Pisabarro MT, Buchholz F (2021). Pairing of single mutations yields obligate Cre-type site-specific recombinases. Nucleic Acids Res. 2021 Dec 24:gkab1240. doi: 10.1093/nar/gkab1240. Online ahead of print. PMID: 34951450. PubMed

Camgoz A, Paszkowski-Rogacz M, Satpathy S, Wermke M, Hamann MV, von Bonin M, Choudhary C, Knapp S, Buchholz F (2018). STK3 is a therapeutic target for a subset of acute myeloid leukemias. Oncotarget. 2018 May 22;9(39):25458-25473. doi: 10.18632/oncotarget.25238. eCollection 2018 May 22. PubMed

Mircetic J, Dietrich A, Paszkowski-Rogacz M, Krause M, Buchholz F (2017). Development of a genetic sensor that eliminates p53 deficient cells. Nat Commun. 2017 Nov 13;8(1):1463. doi: 10.1038/s41467-017-01688-w. PubMed

Karpinski J, Hauber I, Chemnitz J, Schäfer C, Paszkowski-Rogacz M, Chakraborty D, Beschorner N, Hofmann-Sieber H, Lange UC, Grundhoff A, Hackmann K, Schrock E, Abi-Ghanem J, Pisabarro MT, Surendranath V, Schambach A, Lindner C, van Lunzen J, Hauber J, Buchholz F (2016). Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity. Nature Biotechnology. On-line Feb 22. doi: 10.1038/nbt.3467. PubMed

Gebler C, Lohoff T, Paszkowski-Rogacz M, Mircetic J, Chakraborty D, Camgoz A, Hamann MV, Theis M, Thiede C, Buchholz F (2016). Inactivations of cancer mutations utilizing CRISPR/Cas9. JNCI. doi: 10.1093/jnci/djw183. PubMed

Ding L, Paszkowski-Rogacz M, Winzi M, Chakraborty D, Theis M, Singh S, Ciotta G, Poser I, Roguev A, Chu WK, Choudhary C, Mann M, Stewart AF, Krogan N, Buchholz F (2015). Systems Analyses Reveal Shared and Diverse Attributes of Oct4 Regulation in Pluripotent Cells. Cell Systems. Aug 26;2(1):141-51. doi: 10.1016/j.cels.2015.08.002. PubMed

Ross K, Sedello AK, Putz G, Paszkowski-Rogacz M, Bird AW, Hubner N, Mann M, Waskow C, Stocking C, Buchholz F (2012). Polycomb group ring finger 1 cooperates with Runx1 in regulating differentiation and self renewal of hematopoietic cells. Blood, 3;119(18):4152-61. PubMed

Chakraborty D, Kappei D, Theis M, Nitzsche A, Ding L, Paskowski-Rogacz M, Surendranath V, Berger N, Schulz H, Saar K, Huebner N, Buchholz F (2012). Combined RNAi and localization for functionally dissecting long noncoding RNAs. Nature Methods, 12:9(4):360-2. PubMed

Krastev DB, Slabicki M, Paszkowski-Rogacz M, Hubner NC, Junqueira M, Shevchenko A, Mann M, Neugebauer KM, Buchholz F (2011). A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. Nat Cell Biol. Jun 5;13(7):809-18. doi: 10.1038/ncb2264. PubMed

Slabicki MM, Theis M, Krastev DB, Samsonov EM, Junqueira M, Paszkowski-Rogacz M, Teyra J, Heninger AK, Poser I, Prieur F, Truchetto J, Confavreux C, Marelli C, Durr A, Camdessanche JP, Brice A, Shevchenko A, Pisabarro MT, Stevanin G, Buchholz F (2010). A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. PLoS Biology, 29;8(6):e1000408. PubMed

Ding L, Paszkowski-Rogacz M, Nitzsche A, Slabicki MM, Heninger AK, Kittler R, Junqueira M, Shevchenko A, Schulz H, Hubner N, Doss MX, Sachinidis A, Hescheler J, Caldarelli A, Poser I, Theis M, Buchholz F (2009). A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell, 8;4(5):403-15. PubMed

Poser I, Sarov M, Hutchins J, Heriche JK, Toyoda Y, Nitzche A, Pozniakovsky A, Hegemann B, Bird A, Pelletier L, Kittler R, Naumann R, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA (2008). BAC TransgeneOmics: A high-throughput method for exploration of protein function in mammals. Nature Methods, 5(5):409-15. PubMed

Sarkar I, Hauber I, Hauber J, Buchholz F (2007). HIV-1 Proviral DNA Excision Using an Evolved Recombinase. Science, 29;316(5833):1912-5. PubMed

Kittler R, Putz G, Pelletier L, Poser I, Heninger AK, Drechsel D, Fischer S, Konstantinova I, Habermann B, Grabner H, Yaspo ML, Himmelbauer H, Korn B, Neugebauer K, Pisabarro MT, Buchholz F (2004). An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. Nature 432 , 1036-1040. PubMed